Additional module information: Modulbeschreibung des Moduls Molekulare Mikrobiologie und Mikrobenphysiologie

UN Sustainable Development Goals (SDGs): 3, 6, 14, 15

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Content:
Lecture:
Bacterial adaptation mechanisms to stress, starvation and other growth-limiting conditions, microbial biofilms, microbial redox physiology, signal transduction and regulation of gene expression, mechanisms of microbial pathogenicity, protein quality control.

Practical course:
Regulation of gene expression after stress (reporter genes lacZ fusions, ß-galactosidase determination; Northern blot analyses) Molecular biology/genetic engineering (PCR, cloning, blue/white screening in E. coli, mutant construction in B. subtilis, overexpression and Affinity purification of recombinant proteins in E. coli, measurement of enzyme activities of redox-sensitive recombinant proteins (GapDH), measurement of roGFP2 redox sensors.

Seminar:
Theoretical and methodological introduction to the internship experiments, presentation of results from the internship experiments and their critical discussion, taking into account generally accepted theories and findings as well as the methods and techniques used.


Qualification goals:
The module provides a wide range of in-depth knowledge of microbial stress physiology and adaptation mechanisms of bacteria to environmental changes, including sporulation, specific and general stress responses and stationary phase processes. The students will learn about the basics of bacterial transcription and the role of transcriptional and post-transcriptional mechanisms of gene regulation in bacteria. In the practical work, methods of genetics, molecular biology and microbial physiology will be applied to study mechanisms of bacterial stress responses. After completing the module, the students are able to formulate specific research questions and apply experimental approaches to analyze bacterial gene regulatory mechanisms.

The exam on the content of V, S, Ü+P will take place in the last week on February 6th, 2026.

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1. FUCHS, G. (Hrsg.) Allgemeine Mikrobiologie, begründet von Hans G. SCHLEGEL, 9., vollständig überarbeitete und erweiterte Auflage 2014, Georg Thieme Verlag, Stuttgart, New York.
2. MUNK, K. (Hrsg.) Taschenlehrbuch Biologie: Mikrobiologie. 1. Auflage, Georg Thieme Verlag, 2008, Stuttgart.
3. MADIGAN, M. T., J. M. MARTINKO, D. A. STAHL and D. P. CLARK. Brock Biology of Microorganisms, 13th edition, Pearson Education, Inc., 2012, San Francisco.
4. MADIGAN, M. T., J. M. MARTINKO, D. A. STAHL und D. P. CLARK. Brock Mikrobiologie, 13., aktualisierte Auflage, Pearson Higher Education, 2013, München.
5. Lottspeich, F. und J.W.Engel (Hrsg) Bioanalytik. 2.Auflage, Spektrum, Akademischer Verlag, Elsevier GmbH, München, 2006
6. Special literature reviews to the topics are made available Schließen