216404a
Seminar
Seminar on Bioanalytical Mass Spectrometry / Proteomic Analysis
Benno Kuropka
Comments
- Fundamentals of proteome analysis
- Technical basics of a mass spectrometer
- Sample preparation and chromatographic peptide separation
- Ionization methods (ESI and MALDI)
- Protein identification by MALDI peptide mass fingerprinting
- Methods for protein analysis (top-down, bottom-up, targeted)
- Fragmentation methods and interpretation of MS/MS spectra for sequence determination
- Methods of quantitative proteome analysis including different labeling techniques
- Identification of protein interactions by affinity-purification MS
- Analysis of non-covalent protein interactions using native MS
- Guest Seminars on different topics: e.g. MALDI imaging, crosslinking-MS, carbohydrate analysis, metabolomics
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