60100613 Lab Seminar

WiSe 20/21: Current topics in cell physiology

Dorothee Günzel

Additional information / Pre-requisites

Please bring lab coat, if available!


Block course during the semester break. The exact time will be determined by doodle (Duration: 2 weeks; all day)

Location: Charité Campus Benjamin Franklin (Steglitz, Hindenburgdamm 30), Institut für Klinische Physiologie

For further information: http://klinphys.charite.de/bioinfo/

or mail to Dorothee Günzel

Within this course you will generate structural models of proteins by homology modelling. You will develop hypotheses which amino acids should be decisive for the structure.  These Hypotheses will be tested by carrying out molecular biologic experiments (such as site-directed mutagenesis by using two-step PCR). The construct will be cloned into expression vectors, transformed and amplified in bacteria, extracted, sequenced and overexpressed in cultured cells.

These cells will be analyzed in the confocal laser scanning microscope and by other techniques. The results will be evaluated and interpreted in the context of the original hypitheses.

The experimental part will be flanked by seminars introducing the theoretical background and the various techniques.

The exact program of this course depends on the actual research of the institute and is tightly connected to our actual projects.


Suggested reading

Milatz S, Piontek J, Hempel C, Meoli L, Grohe C, Fromm A, Lee IM, El-Athman R, Günzel D (2017) Tight junction strand formation by claudin-10 isoforms and claudin-10a/-10b chimeras. Ann. N.Y. Acad. Sci. 1405: 102-115 (https://www.ncbi.nlm.nih.gov/pubmed/28633196)

Piontek J, Winkler L, Wolburg H, Müller SL, Zuleger N, Piehl C, Wiesner B, Krause G, Blasig IE (2008) Formation of tight junction: determinants of homophilic interaction between classic claudins. FASEB J. 22: 146-158 (https://www.ncbi.nlm.nih.gov/pubmed/17761522)



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